COLD HEMAGGLUTINATION AND COLD HEMOLYSIS. THE HEMOLYSIS PRODUCED BY SHAKING COLD AGGLUTINATED ERYTHROCYTES

The Mechanism of Hemolysis in Paroxysmal Cold

Donath and Landsteiner demonstrated that the abnormal factor responsible for hemolysis in paroxysmal cold hemoglobinuria (PCH) is in the patient's serum (1). Inactivation of the serum by heat prevented hemolysis, but the addition of fresh serum restored the activity. The hemolytic reaction in vitro was shown to occur in two phases: 1) a cold phase during which antibody (hemolysin) is adsorbed o...

The variability of hemolysis in the cold agglutinin syndrome.

The amount of lysis effected by cold agglutinins is directly related to the ability of the antibody to initiate complement activation. This ability is modified by the concentration of antibody, its thermal amplitude (the highest temperature at which the antibody will react with the cell), the degree to which antibody fixation is modified by the presence of complement components (particularly C3...

Chronic hemolytic anemia due to cold agglutinins: the mechanism of resistance of red cells to C' hemolysis by cold agglutinins.

The red cells of patients with chronic hemolytic anemia due to cold agglutinins are agglutinated by antiglobulin serum in a nongamma reaction due to the coating of beta-globulins, C'4 and C'3. The red cells of such patients are abnormally resistant to C' hemolysis by cold agglutinin. Normal red cells can be made equally resistant to C' hemolysis by incubation with cold agglutinin and normal ser...

Bacterial hemagglutination and hemolysis.

Hot and Cold Tensile Behavior of Al 6061 Produced by Equal Channel Angular Pressing and Subsequent Cold Rolling

The full annealing AA6061 aluminum alloy was subjected to severe plastic deformationvia the combination of equal channel angular pressing (ECAP) and cold rolling (CR) in order torefine its microstructure and to improve its mechanical properties. According to the results of hotand cold tensile tests, the combination of ECAP and CR significantly affected the final strengthand ductility of studied...